万建民团队揭示OsCNGC9蛋白在水稻抗病中的作用

来源：植物科学最前沿

近日，Cell Research（IF15.393）在线发表了中国农科院作物科学研究所万建民团队题为“A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice”的研究论文，揭示了环核苷酸门控离子通道蛋白9（OsCNGC9）在介导细胞质钙离子升高和水稻抗病中的作用。

该研究团队通过突变体鉴定和遗传分析等方法克隆获得水稻CDS1（CELL DEATH and SUSCEPTIBLE to BLAST 1）基因编码的环核苷酸门控离子通道蛋白OsCNGC9，该基因对稻瘟病抗性具有正向的调控作用。OsCNGC9能够介导PAMP诱导的钙离子流，这对于PAMP触发的ROS爆发和PTI相关防御基因表达具有重要作用。进一步结果表明，PTI相关的受体样胞质激酶OsRLCK185能够与OsCNGC9互作，并通过磷酸化修饰改变其通道活性。

Fig. 7 A proposed model for OsCNGC9 in regulating rice PTI.PAMPs, such as the flg22 and chitin, are sensed by pathogenrecognize receptors (PRRs), leading to the activation of OsRLCK185by OsCERK1.12,13 Subsequently, the activated OsRLCK185 phosphorylatesOsCNGC9 to enhance its channel activity. The activatedOsCNGC9 induces extracellular Ca2+ influx and then triggers ROSburst and PTI-related gene expressions, which ultimately leads toenhanced disease resistance in rice. “OsCNGC9/?” represents thepotential homomeric and/or heteromeric tetramers that containsOsCNGC9

论文链接：

https://www.nature.com/articles/s41422-019-0219-7