朱健康团队创建高效的基因定点插入和替换技术

来源：植物科学最前沿

7月6日，Nature Biotechnology 杂志在线发表了朱健康院士团队题为“Targeted, efficient sequence insertion and replacement in rice”的研究论文，在水稻中创建了高效的基因定点插入和替换技术。

AbstractCRISPR–Cas9 methods have been applied to generate random insertions and deletions, large deletions, targeted insertions or replacements of short sequences, and precise base changes in plants. However, versatile methods for targeted insertion or replacement of long sequences and genes, which are needed for functional genomics studies and trait improvement in crops, are few and largely depend on the use of selection markers8,9,10,11. Building on methods developed in mammalian cells12, we used chemically modified donor DNA and CRISPR–Cas9 to insert sequences of up to 2,049 base pairs (bp), including enhancers and promoters, into the rice genome at an efficiency of 25%. We also report a method for gene replacement that relies on homology-directed repair, chemically modified donor DNA and the presence of tandem repeats at target sites, achieving replacement with up to 130-bp sequences at 6.1% efficiency.原文链接：
https://www.nature.com/articles/s41587-020-0581-5